Genomic FRT-like sequences can have many opportunity upstream and downstream TAL recognition sequences considering that the requirements for that TAL recognition sequences are fairly calm: the only real major prerequisite is usually a thymine on the place N-1 of your sequence, 5′T38. We hypothesized the TAL modules have to acknowledge DNA sequence of 15–24 bp in duration to be valuable in targeting Flp-TAL recombinases to the desired FRT-like sequence also to be easily manageable through the technical standpoint.
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